Journal: The Journal of Immunology Author Choice

Article Title: The Metalloproteinase ADAMTS5 Is Expressed by Interstitial Inflammatory Cells in IgA Nephropathy and Is Proteolytically Active on the Kidney Matrix

doi: 10.4049/jimmunol.2000448

Figure Lengend Snippet: Identification of matrix proteins by urine proteomics leads to the discovery of ADAMTS5 in IgAN kidneys. ( A ) LC-MS/MS proteomics of healthy donor and IgAN patient urine identified multiple ECM proteins present in IgAN urine (see Ref. ). Matrix proteins were selected using gene ontology (BinGO) and are presented as a protein-protein interaction network. Yellow and red proteins are increased in IgAN urine, gray are unchanged, and blue are decreased in comparison with healthy samples. Proteomics analysis including all identified proteins is described in detail in . ( B ) The mRNA expression of ADAMTS5 and TIMP3 was examined in a published microarray dataset comparing 25 IgAN patients with six healthy donor kidney biopsies. Microarray gene expression was retrieved directly from National Center for Biotechnology Information–Gene Expression Omnibus (GSE35488; https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE35488 ). Microarray data are available in . ADAMTS5 and TIMP3 were significantly regulated in IgAN . Differences were examined using standard t test using data curated and processed by NCBI-GEO as described in GSE35488; https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE35488 . The p values are shown. ( C ) ADAMTS5 protein immunofluorescence was measured in 13 healthy (kidney transplant donors) versus 20 IgAN patients. IgAN donors were biopsy diagnosed. ADAMTS5 staining was quantified using ADAMTS5-stained particle counting corrected to biopsy tissue area on ImageJ and values were compared using two-tailed t test. ( D ) For the 20 IgAN patients, ADAMTS5 immunofluorescence was correlated to their matching urine protein concentration (proteinuria; mg/dl) and eGFR (estimated glomerular filtration rate; ml/min/1.73 m 2 ) values. The r and p values were computed using nonparametric Spearman test given the dissimilar nature of the correlated values. ( E ) ADAMTS5 staining was also compared in the same IgAN biopsies but biopsies were grouped according to their histological MEST-C score. We focused on the (T) score (percentage of biopsy area affected by tubular atrophy or interstitial fibrosis, whichever is greater) and compared less affected T0 biopsies ( n = 7) with more affected T1/T2 biopsies ( n = 13). There was a significant increase in ADAMTS5 staining in T1/T2 specimens ( t test). ( F ) ADAMTS5 biopsy staining was also measured separately in the tubulointerstitium and glomeruli and compared in healthy versus IgAN biopsies. ADAMTS5 staining in the IgAN interstitium ( IgAN Interst ) is significantly increased in comparison with staining in IgAN glomeruli ( IgAN Glom ), as well as in comparison with healthy interstitium and glomeruli, which are NS different to each other (ANOVA with Fisher least significant difference, multiple comparison test). ( G and H ) Examples of ADAMTS5 immunofluorescence in IgAN and healthy kidney biopsies costained with relevant proteins. In (G) ADAMTS5 (red) was costained with ADAMTS1 (green) and TIMP3 (blue). TIMP3 staining was below the threshold of confident detection in either IgAN or healthy biopsies. In (H) ADAMTS5 (red) was costained with TIMP1 (green). ADAMTS5 is close but does not appear to colocalise with TIMP1 in affected tubulointerstitial areas. tub denotes renal tubules and glom denotes glomeruli. Note that ADAMTS5 is increased in areas of tubulointerstitial inflammatory infiltration and remodelling. IgAN glomeruli also contain ADAMTS5 + cells. In healthy biopsies, there were sporadic ADAMTS5-stained (red) cells in the interstitium [(G) healthy] and glomeruli [(H) healthy]. White-dashed boxes denote areas shown in higher magnification in lower panels.

Article Snippet: Sections were treated using acid-based Ag retrieval and dewaxing solution (Abcam) at 98°C for 1 h. Sections were thoroughly washed, blocked in 10% normal donkey serum (Dako) for 1 h, and subsequently incubated with anti-human primary Abs against the following proteins: ADAMTS5 (raised in rabbit), CD64, and vimentin (mouse) from Abcam and TIMP3 (mouse), myeloperoxidase, TIMP1, lumican (LUM), ADAMTS1, versican (VCAN), collagen-4, and CD206 (all goat) from Bio-Techne.

Techniques: Liquid Chromatography with Mass Spectroscopy, Comparison, Expressing, Microarray, Immunofluorescence, Staining, Two Tailed Test, Protein Concentration, Filtration

Journal: Heliyon

Article Title: Epimedin C alleviated osteoarthritis development by regulating chondrocyte Nrf2-mediated NLRP3 inflammasome axis

doi: 10.1016/j.heliyon.2024.e40458

Figure Lengend Snippet: Effect of Epi C on ECM degradation in IL-1β-induced chondrocytes. (A) Western blot assays were used to determine the protein levels of Aggrecan, Collagen II, MMP13 and ADAMTS5 (n = 3 for each group). (B) The mRNA expressions of Aggrecan, Collagen II, MMP13 and ADAMTS5 were detected by RT-qPCR (n = 3 for each group). (C) Representative images of Collagen II immunofluorescence staining. Data represented the mean ± SEM from three independent experiments. ## p < 0.01, ### p < 0.001 vs. Control group; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 vs. IL-1β group.

Article Snippet: Antibodies are as follows: Aggrecan (1:1000, Abcam, ab313636), Collagen II (1:1000, Abcam, ab307674), MMP13 (1:1000, Cell signaling technology, #69926), ADAMTS5 (1:1000, Cell signaling technology, #12897), iNOS (1:1000, Cell signaling technology, #13120), COX-2 (1:1000, Cell signaling technology, #12282), Nrf2 (1:1000, Cell signaling technology, #12721), SOD-2 (1:1000, Cell signaling technology, #13141), NQO1 (1:1000, ABclonal, A23486), HO-1 (1:1000, Cell signaling technology, #70081), NLRP3 (1:1000, Cell signaling technology, #15101), ASC (1:1000, Abcam, ab283684), Pro-Caspase-1 (1:1000, Cell signaling technology, #24232), Caspase-1 P10 (1:1000, abcam, ab179515), IL-1β (1:1000, Cell signaling technology, #12242), GAPDH (1:1000, Cell signaling technology, #2118) and Rabbit anti-goat secondary antibody (1:1000, ABclonal, AS014).

Techniques: Western Blot, Quantitative RT-PCR, Immunofluorescence, Staining, Control

Journal: Heliyon

Article Title: Epimedin C alleviated osteoarthritis development by regulating chondrocyte Nrf2-mediated NLRP3 inflammasome axis

doi: 10.1016/j.heliyon.2024.e40458

Figure Lengend Snippet:

Article Snippet: Antibodies are as follows: Aggrecan (1:1000, Abcam, ab313636), Collagen II (1:1000, Abcam, ab307674), MMP13 (1:1000, Cell signaling technology, #69926), ADAMTS5 (1:1000, Cell signaling technology, #12897), iNOS (1:1000, Cell signaling technology, #13120), COX-2 (1:1000, Cell signaling technology, #12282), Nrf2 (1:1000, Cell signaling technology, #12721), SOD-2 (1:1000, Cell signaling technology, #13141), NQO1 (1:1000, ABclonal, A23486), HO-1 (1:1000, Cell signaling technology, #70081), NLRP3 (1:1000, Cell signaling technology, #15101), ASC (1:1000, Abcam, ab283684), Pro-Caspase-1 (1:1000, Cell signaling technology, #24232), Caspase-1 P10 (1:1000, abcam, ab179515), IL-1β (1:1000, Cell signaling technology, #12242), GAPDH (1:1000, Cell signaling technology, #2118) and Rabbit anti-goat secondary antibody (1:1000, ABclonal, AS014).

Techniques: Sequencing